Primer

Part:BBa_K4686020:Design

Designed by: Maia Weatherly   Group: iGEM23_Princeton   (2023-10-08)


SNIPR Reporter Backbone Reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primers were designed to PCR the backbone and linearize it, so that it would have overhangs for future use in Gibson assembly


Source

The primer was designed by a member of our team. the piggybac vector as the pBR70 plasmid was given to us by our mentor, Beatrice Ramm, who sourced it from HeraBiolabs https://www.herabiolabs.com/piggybac-products/

References